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Mapping a Bacterial Chromosome

Chapter 8Case History: Mapping a Bacterial ChromosomeBackgroundConjunction mapping can be used to locate genes on a bacterial chromosome. In this example, the F+ strain is able to synthesize methionine, valine, leucine, and histidine. The F- strain is unable to synthesize these amino acids; therefore, they must be supplied in the growth medium. Use the results from replica-plating to answer the questions.Questions 1. On which media, if any, should the F+ be able to grow? The F-?2. Identify the prototrophs and auxotrophs. How can you tell?3. Can you map the chromosome of this species? What further information do you need?

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